The effects of Akt inhibitor on selenium-mediated inhibition of cell viability and PSA expression. A. Cell viability in LNCaP and C4-2 cells pre-treated with an Akt-specific inhibitor (Akti, 2 μM) for 1 h and/or treated with selenium (10 μM SM or p-XSC) for 1.5 h was measured by MTT assay. Results are expressed as percent of vehicle-only treated control. (*p<0.05, **p<0.001) B. PSA mRNA levels in LNCaP and C4-2 cells pre-treated with an Akt-specific inhibitor (Akti, 2 μM) for 1 h and/or treated with p-XSC (10 μM) for 1.5 h and stimulated with 10 nM DHT were measured by QRT-PCR. Results are normalized to GAPDH mRNA levels and are expressed as percent of vehicle-only treated control. (*p<0.05, **p<0.01, ***p<0.001)