Skip to main content
. 2010 May 7;285(34):25913–25927. doi: 10.1074/jbc.M110.139485

FIGURE 1.

FIGURE 1.

HPASMC expresses both ETa and ETb receptors. A, Q-PCR of 10 ng of 1st strand cDNA from HPASMC probed for the ETa receptor and ETb receptors with comparative Ct (ΔCt) to the β2-microglobulin gene. B, Western blot analysis of 20 μg of total cellular extracts (T) and membrane extracts (M) from HPASMC probed with rabbit anti-ETa and rabbit anti-ETb antibodies. ET-1 causes a concentration-dependent increase in the synthesis of cAMP that is more effectively antagonized by the ETa-specific antagonist BQ123 and the dual specific ETa/ETb antagonist bosentan. C, a concentration range of ET-1 induces cAMP synthesis in HPASMC. D, HPASMC were treated with a concentration curve of ETa antagonist (BQ123) (▾), ETb antagonist (BQ788) (▴), and dual specificity ETa/ETb antagonist (bosentan) (■) for 30 min prior to the 20-min stimulation of cAMP synthesis by ET-1 at 1 × 10−7 m. All measurements represent the mean ± standard error of three independent experiments.