TABLE 2.
Complementation of E. coli ΔhemG with various expression plasmids
Plasmids for recombinant protein expression in E. coli were transformed into the hemG-deficient strain SASX38 (22). Positive complementation resulted in the growth of wild-type sized E. coli colonies after overnight incubation at 37 °C.
| Protein expressed from plasmid | Complementation |
|---|---|
| pTrcHisA (empty vector) | − |
| Human protoporphyrinogen oxidase | + |
| S. coelicolor HemY | − |
| S. coelicolor HemY + HemH | − |
| S. coelicolor HemY + HemQ | − |
| S. coelicolor HemY + HemH + HemQ | + |
| M. tuberculosis HemY | − |
| M. tuberculosis HemY + HemH | − |
| M. tuberculosis HemY + HemQ | − |
| M. tuberculosis HemY + HemH + HemQ | + |
| P. acnes HemY | − |
| P. acnes HemY + HemH/Q | + |
| B. subtilis HemY | − |
| B. subtilis HemY + HemH | − |
| B. subtilis HemY + HemQ | − |
| B. subtilis HemY + HemH + HemQ | + |
| P. acnes HemY + M. tuberculosis HemH | − |
| P. acnes HemY + M. tuberculosis HemQ | − |
| P. acnes HemY + M. tuberculosis HemQ +HemH | + |
| P. acnes HemH/Q and M. tuberculosis HemY | + |
| M. tuberculosis HemY + HemH + B. subtilis HemQ | + |
| M. tuberculosis HemH + HemQ + B. subtilis HemY | + |
| M. tuberculosis HemY +HemQ + B. subtilis HemH | + |
| B. subtilis HemY + HemH + M. tuberculosis HemQ | + |