FIGURE 2.

Detection of SR proteins involved in RA-mediated PKCδVIII expression. NT2 cells were treated with RA (10 μm) for 24 h or without RA (control), and Western blot analysis was performed on whole cell lysates using (a) mAb104 antibody that detects all SR proteins and (b) specific antibodies as indicated in the figure. Molecular masses are indicated (kDa). Gels are representative of three separate experiments, and results indicate that SC35 may be involved in increased expression of PKCδVIII by RA. Results demonstrate an increase in SC35 levels concurrent with an increase in PKCδVIII expression upon RA treatment.