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Repression and antirepression of in vitro transcription of irr. In vitro transcription initiated from the irr promoter was carried out using E. coli RNA polymerase in the presence of MnCl2 and the presence (+) or absence (−) of 10 nm Fur or 150 nm Irr. The 157-nucleotide radiolabeled RNA product was visualized on a gel by autoradiography.
