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Wnt3a and heparin synergistically enhanced ALP activity. MC3T3-E1 (A) or C2C12 (B) cells seeded at 30,000 cells/cm2 or rOB cells (C) seeded at 5000 cells/cm2 were treated with either 50 ng/ml Wnt3a, increasing doses of heparin (0.5 to 50 μg/ml) or the combination of 50 ng/ml Wnt3a with indicated doses of heparin. Untreated samples served as the control. ALP activity was determined 72 h after treatment.
