FIGURE 5.

Substrate specificity of wild-type Hxt7 and D340C of Hxt7, D340C of Cys-less Hxt7, and Cys-less Hxt7. Transport activities were measured at 30 °C for 5 s with 0.1 mm d-[¹⁴C]glucose as substrate in the presence of a 200-fold excess of the indicated nonradioactive sugars. Glucose transport activity for each transporter was expressed as a percentage of that determined in the presence of 20 mm sorbitol. Data are means ± S.E. (n ≥ 4). 2DG, 2-deoxy-d-glucose; d-Glc, d-glucose; Fru, d-fructose; Man, d-mannose; 3OMG, 3-O-methyl-d-glucose; Xyl, d-xylose; 6DG, 6-deoxy-d-glucose; Gal, d-galactose; Allose, d-allose; l-Glc, l-glucose.