Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Jun 16;285(34):26384–26389. doi: 10.1074/jbc.M110.115915

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 2. — Characterization of the genome integration-free 2A-3FiPS clones. A, morphology of three genome integration-free 2A-3FiPS colonies. B, immunocytochemical detection of Oct3/4 using the h-134 antibody (Santa Cruz Biotechnology). 4′,6-Diamidino-2-phenylindole (DAPI) was used as a nuclear counter stain. C, RT-PCR analysis of ES-marker genes. Error bars indicate standard deviations (n = 3). D, methylation analysis of the Oct3/4 and Nanog gene promoter regions using bisulfite genomic sequencing. Open and closed circles indicate unmethylated and methylated CpGs, respectively. E, teratomas derived from the 3 2A-3FiPS clones. F, a chimeric mouse generated from 2A-3FiPS-1, -3, and -4.