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. 2010 Jun 16;285(34):26451–26460. doi: 10.1074/jbc.M110.112979

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Topo IIβ is down-regulated in concert with SP120. A, cerebellar granule cells treated with siRNAs for topo IIβ, SP120, or control (mock) at day 0 were cultured for 2 days. Levels of proteins designated were estimated by Western blotting. A nuclear matrix protein, matrin 3, was detected with a rabbit polyclonal antibody and used as a loading control. B, band densities on immunoblots were measured and plotted in the graph after correction of loading errors and normalization by the mock. C, cerebellar granule cells treated with siRNA at day 0 were cultured for 5 days. RNA samples were prepared, and mRNA levels for topo IIβ and β-actin were determined by RT-qPCR. Copy numbers of mRNA in 20 ng of total RNA were calculated and plotted in the graph. The data represent average ± S.D. of triplicate analysis. D, protein samples from the same set of cells used in C were analyzed by Western blotting.