FIGURE 4.

DNA repair efficiency of Cernunnos mutants. A and D, survival of Cernunnos-deficient cells, transduced with the pMND vector containing WT or the indicated Cernunnos mutants, 14 days after bleomycin treatment is shown. B and E, survival of empty MND vector, WT or the indicated mutant Cernunnos, complemented Cernunnos-deficient cells, without any treatment. Cells were followed for 57 days. C and F, percentage of IRIF-positive cells is shown. 40 transduced cells were scored for DAPI and γH2AX foci and IR-untreated, 2 and 24 h after IR (2 Gray). A cell was considered IRIF-positive if it had >10 IRIFs.