FIGURE 5.
CD44 expression is enhanced in aortas of apoE−/− mice, whereas HA expression was up-regulated in atherosclerotic lesions of apoE−/− mice as compared with apoE−/−/p47phox−/− mice. A, CD44 expression was measured in protein lysates from aortas of apoE−/− and apoE−/−/p47phox−/− mice using CD44 ELISA. Data expressed are mean ± S.E. of five mice in each group (*, p < 0.001). B, representative aortic root sections from apoE−/− and apoE−/−/p47phox−/− mice were stained using biotinylated HABP or oil red O. Localization of smooth muscle cell was shown by staining for α-SM actin. C, expression of HA in atherosclerotic lesions, determined by immunohistochemistry scoring. Data are mean ± S.E. (n = 6, *, p < 0.05).