TABLE 2.

Solution binding measurements of human and rat Shh to ch5E1 Fab by isothermal titration calorimetry

Hh ligand	Divalent metal cation	KD	ΔH	TΔS	ΔG
		nm	kcal/mol	kcal/mol	kcal/mol
Shh	None	7	−13.6	2.3	−11.3
Shh	Zn2+	1.1	−18.8	6.4	−12.4
Shh	Ca2+	1.8	−14.7	2.6	−12.1
Shh	Zn2+, Ca2+	0.7	−19.6	6.9	−12.7
Rat Shha	Zn2+, Ca2+	1.1	−12.0	0.4	−12.4

^a Rat Shh refers to the sequence as described in Roelink et al. (43).