FIGURE 1.

Protein synthesis is required for TGF-β1-mediated changes in cell morphology and Rho activation. Serum-starved ARPE-19 cells were treated with 10 ng/ml TGF-β1 for 0, 24, or 48 h in the presence or absence of the protein synthesis inhibitor cycloheximide (CHX). D, DMSO. A, the cells were monitored by phase-contrast microscopy at the indicated times. B, shown is a Western blot of N-cadherin under the same treatment conditions as A. C, the actin cytoskeleton was visualized using rhodamine-labeled phalloidin; blue is from Hoechst staining of the nuclei to show all cells. Bar, 20 μm. D, lysed cells were subjected to GST pulldown assays using GST-rhotekin and Western blot analysis using anti-Rho.