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Bar graph for the apparent free energy (ΔG2app) of calcium binding to CaM. ΔG2app of calcium binding to WT CaM1–80 and WT CaM1–148 at sites I and II (A) and ΔG2app of calcium binding to WT CaM76–148 and WT CaM1–148 at sites III and IV (B) with (dashed bars) and without (open bars) CaMKIIp.
