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Equilibrium calcium titrations of site I (E31Q) and II (E67Q) mutants of CaM1–148. Phenylalanine fluorescence of the N-domain of E31Q (A), E67Q (C) CaM1–148 and tyrosine fluorescence of the C-domain of E31Q (B) and E67Q (D) CaM1–148 were monitored with (■, ●) and without (□, ○) CaMKIIp.
