Table IV.
Effect of CaMKIIp on Free Energies of Calcium Binding to Mutants of CaM1–148
| Protein | Peptide Equivalent | ΔG1app | ΔG2app | ΔGc | ΔΔG2a |
|---|---|---|---|---|---|
| Sites I and II | |||||
| WT CaM1–148 | 0 | −5.98 ± 0.02 | −12.82 ± 0.09 | −1.67 ± 0.08 | -- |
| 5 | −6.00b | −17.98 ± 0.16 | −6.79 ± 0.16 | −5.16 | |
| E31Q CaM1–148 | 0 | −6.00 ± 0.14 | -- | -- | -- |
| 5 | −7.54 ± 0.11 | −15.25 ± 0.13 | −2.39 ± 0.32 | ||
| E67Q CaM1–148 | 0 | -- | -- | -- | -- |
| 5 | −8.21 ± 0.84 | −17.14 ± 0.33 | −1.52 ± 1.28 | -- | |
| E104Q CaM1–148 | 0 | −6.36 ± 0.24 | −13.13 ± 0.04 | −1.21 ± 0.45 | -- |
| 5 | −7.75 ± 0.06 | −14.17 ± 0.11 | 0.51 ± 0.18 | −1.14 | |
| E140Q CaM1–148 | 0 | −5.93 ± 0.46 | −13.13 ± 0.16 | −2.07 ± 0.92 | -- |
| 5 | −7.19 ± 0.19 | −14.52 ± 0.11 | −0.94 ± 0.28 | −1.39 | |
| Sites III and IV | |||||
| WT CaM1–148 | 0 | −6.40 ± 0.19 | −15.06 ± 0.03 | −3.05 ± 0.35 | -- |
| 5 | −6.40c | −18.26 ± 0.05 | −6.27 ± 0.05 | −3.20 | |
| E31Q CaM1–148 | 0 | −5.98 ± 0.18 | −14.88 ± 0.08 | −3.73 ± 0.45 | -- |
| 5 | −7.90 ± 0.15 | −17.38 ± 0.04 | −2.38 ± 0.31 | −2.50 | |
| E67Q CaM1–148 | 0 | −6.14 ± 0.52 | −15.10 ± 0.01 | −3.62 ± 1.04 | -- |
| 5 | −6.79 ± 0.39 | −17.00 ± 0.01 | −4.22 ± 0.79 | −1.90 | |
| E104Q CaM1–148 | 0 | −6.37 ± 0.05 | -- | -- | -- |
| 5 | −7.97 ± 0.04 | −16.53 ± 0.01 | −1.39 ± 0.07 | ||
| E140Q CaM1–148 | 0 | −5.39 ± 0.11 | -- | -- | -- |
| 5 | −6.38 ± 1.06 | −14.57 ± 0.02 | −2.60 ± 2.11 | ||
a
ΔΔG2=ΔG2app (CaM+CaMKIIp) − ΔG2app (CaM)
b
ΔG1 for N was fixed to −6.00 kcal/mol while ΔG1 of C was fixed to −6.40 kcal/mol.
c
ΔG1 of C was fixed to −6.40 kcal/mol.