Skip to main content
. 2010 Aug 19;6(8):e1001072. doi: 10.1371/journal.pgen.1001072

Figure 2. Checkpoint response to telomere uncapping in the absence of Sgs1, Exo1, and Rad9.

Figure 2

(A) Yeast strains of the indicated genotypes (all with cdc13-1 cdc15-2 bar1 mutations) were arrested in G1 at 23°C with α factor and released into 36°C, cells were collected at the indicated time points, and scored for the percentage of cells arrested in medial nuclear division using DAPI staining. (B) As in (A) but the percentage of cells arrested in late nuclear division were quantified. (C) Yeast strains of the indicated genotypes (all with cdc13-1 cdc15-2 bar1 mutations) were arrested in G1 at 23°C and released into 36°C for up to two hours. Western blot were first probed with anti-Rad53 antibody, membranes were stripped and reprobed with anti-tubulin antibody.