Figure 3. Resection of uncapped telomeres in the absence of Sgs1, Exo1, and Rad9.

(A–H) A series of yeast strains with the indicated genotypes (all with cdc13-1 cdc15-2 bar1 mutations) were arrested in G1 at 23°C and released into 36°C to induce telomere uncapping, the amount of ssDNA at the TG strands at two repetitive telomeric locus, Y'600 and Y'5000 and two single copy loci, YER188W and PDA1 were measured by QAOS as described previously [41]. The values plotted are the mean value ± SD.