Figure 4. Cellular response to long term absence of Cdc13.

(A) Yeast strains of the indicated genotype were streaked onto YPD agar plates and the plates were incubated at 23°C or 36°C for three days. The strains were then photographed and restreaked onto another YPD plate. This cycle is repeated for nine times (p = passage). (B) DNA was purified from the strains in (A) or a wild-type strain following further incubation in liquid culture for 48 hours at 36°C. Southern blots were performed as in Figure 1C.