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. 2010 Aug 19;6(8):e1001049. doi: 10.1371/journal.ppat.1001049

Figure 6. Mechanism of reduced rgRSV infection of A549 cells in relation to GAG expression.

Figure 6

A549, CHO K-1, CHO pgsA-745 or CHO pgsD-677 cells were infected with rgRSV after pre-incubation of the cells (black symbols) or the virus (white symbols) with Pam3CSK4 (10 µg/ml) for 30 minutes at 37°C. In the GAG-expressing cell lines A549 and CHO-K1 pre-incubation of cells with Pam3CSK4 resulted in reduction of RSV infection, but in the GAG-deficient cell lines CHO-pgsA745 and CHO-pgsD677 this same condition resulted in enhancement of infection (p<0.01 in multiple Student's t-test with Bonferroni correction). Data are presented as percentages GFP-positive cells (means ± SD of triplicates). A representative experiment out of three is shown.