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. 2010 Aug 19;6(8):e1001060. doi: 10.1371/journal.ppat.1001060

Figure 2. TviA represses flagellar gene expression under conditions mimicking tissue osmolarity.

Figure 2

(A) A S. Typhi flhC::lacZYA mutant (SW197, dark gray bars), a S. Typhi ΔviaB flhC::lacZYA mutant (SW186, open bars), a S. Typhimurium flhC::MudJ mutant (SW335, light gray bars) and a S. Typhimurium ΔphoN::tviA flhC::MudJ mutant (SW316, black bars) were grown in tryptone yeast extract medium and β-galactosidase activity was measured. NaCl was added at the concentrations to increase osmolarity. Bars represent the geometric mean of three independent experiments ± standard error. Asterisks indicate statistical significance between data sets: * (P<0.05) or ** (P<0.01). (B) The S. Typhi wild-type strain (Ty2), a ΔviaB mutant (SW347), a ΔtviB-vexE mutant (SW74), and a ΔviaB ΔfliC mutant (SW483) were grown in tissue culture medium (DMEM) and FliC expression was detected by Western blot using H antiserum d. (C) The S. Typhimurium wild-type strain (IR715), a ΔphoN mutant (AJB715), a ΔphoN::tviA mutant (SW474) and a ΔphoN ΔfliC fljB mutant (SW681) were cultured in tissue culture medium (DMEM) and expression of FliC was detected by Western blot using Salmonella H antiserum i. Expression of GroEL was determined to ensure equal loading of samples, (αGroEL). Approximate position of standard proteins with known molecular mass is indicated.