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. 2010 Aug 19;5(8):e12302. doi: 10.1371/journal.pone.0012302

Figure 2. MORN1 knock-out tachyzoites display a lethal phenotype.

Figure 2

(A) Growth curves of the conditional MORN1 knock-out parasites with and without ATc induction displaying a decreased growth rate at 24 hrs post induction. Vacuole sizes of unsynchronized parasites were scored by fluorescence microscopy on fixed parasites using IMC3 antiserum; error bars denote standard deviations of three independent experiments. (B, C) Developmental progression analysis through the tachyzoite division cycle upon 18 hrs (B) and 24 hrs (C) of ATc induction. Developmental stages were defined using centrosome and daughter budding markers as indicated in (D–H). Cultures were unsynchronized and over 100 vacuoles per condition were counted in three independent experiments; error bars denote standard deviation. (D–H) Phenotype development was assessed using centrosome (α-centrin) and cytoskeleton scaffold (α-IMC1) markers as well as DAPI for the nuclear material. Stages were defined as indicated at the bottom right; numbers indicate the number of either centrosomes “C”, daughter buds “B” or nuclei “N”. Stages 2N2C and 2N4C were only detected in the ATc induced samples. Classes shown in (G) and (H) were differentiated for presence of buds as well, but this percentage was very low (1–2%) and is not included in the graphs.