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. Author manuscript; available in PMC: 2010 Aug 19.
Published in final edited form as: Nature. 2010 Mar 25;464(7288):549–553. doi: 10.1038/nature08873

Figure 2. Analysis of coronary vessel sprouting in vitro.

Figure 2

a–e. Intact heart (a) or dissected sinus venosus/atria (SV/A) or ventricle/epicardium (V/Epi) as indicated from e10.5 apelin-nlacZ embryos (or wild type (wt) where noted) cultured for 3 days at 37°C and stained with X-gal (blue nuclei) to show coronary development. Coronary sprouts formed only on intact hearts (a) or when dissected SV/A were recombined with V/Epi (d). Vessels arise from SV/A because no X-gal+ sprouts were detected when SV/A was from wild type (non-transgenic) embryo (e). a’–e’ Close up views. f,f’. Close-up of a cultured intact heart as in a stained for CD31 (f) and with X-gal (f’), confirming lacZ-positive (blue) nuclei are in coronary vessels. Scale bar, 200 μm.