Figure 5. Potency of T cell induction by HPV PsV-encapsidated DNA.
CB6F1/J mice were immunized IVag with 5×107 IU HPV16-PsV-M/M2 or 50 μg naked M/M2 DNA. Negative control mice received 5×107 IU HPV16 PsV-luciferase. All mice were challenged IN with RSV at week 4. DbM187–195 (red) and KdM282–90-specific (blue) CD8+ T cells were detected by tetramer staining in lungs on day 7 post-challenge. * = p≤0.01 and ** = p≤0.001 by one-way ANOVA and T-test compared to empty vector-immunized mice. N=5 mice per group from one independent experiment.