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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Mucosal Immunol. 2010 Jun 16;3(5):475–486. doi: 10.1038/mi.2010.31

Figure 8. HPV PsV encapsidation improves delivery of plasmid DNA.

Figure 8

BALB/c mice were immunized once IVag with a single 5×107 IU dose of HPV16 PsV-M/M2, 5×107 IU HPV45 PsV-M/M2, 5 ng 500 ng, or 50 μg naked M/M2 DNA, or 1μg M/M2 purified protein. Negative control mice received 5×107 IU HPV-luciferase IVag. All mice were challenged IN with RSV at week 4. Seven days after challenge, M and M2-specific CD8+ T cells in lung were measured by tetramer staining (a) and M/M2-specific IgG1 and IgG2 serum responses were measured by kinetic ELISA prechallenge (b) and post-challenge (c). P values represent one-way ANOVA and selected comparisons by T-test (+ = p≤0.05; * ≤0.01; **** ≤0.00001). N=5 mice per group for each postchallenge timepoint from one independent experiment.