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Paromomycin increases inaccuracy of five H245 codons in E. coli. The activity of the near-cognate mutants of H245 is shown in the absence (black) or presence (white) of 200 μg/mL paromomycin. Significant increases in activity (ANOVA, P <0.05) were seen for the two Asp mutants, GAU and GAC, two Arg mutants, CGU and CGC, and the Gln mutant, CAG. The data are represented as in Figure 7.
