FIGURE 3.

Variation in Fluc activity of K529 mutants. The graph shows the Fluc expression of the indicated constructs expressed as a fraction of the expression of wild-type Fluc. The activities for mutants that according to ANOVA are not significantly different from each other or the negative control are shown in black and the two activities that are significantly higher (P <0.05) are shown in white; the negative control UUU mutant is shown in gray. Indicated below each codon is the amino acid it encodes. Error bars are standard error of the mean.