Figure 6.
Endocytosis and gene transfer to freshly excised monkey tracheal explants. Fluid phase endocytosis measured as uptake of FITC-dextran under basal conditions (a), or after addition of 50 nM uPA (b); trachea shows significant background fluorescence. (c) Quantitation of endocytosis (n = 12). X-gal–stained explants infected with Ad/CaPi coprecipitates alone (d) or following treatment with 50 nM uPA (e). (f) Quantitation of gene transfer (n = 4). (g) Thin section of X-gal-stained uPA-treated explant; same explant as shown in d. *P < 0.05. White arrow indicates ciliated cells and yellow arrow indicates nonciliated cell.