Table 1.

Positions, nucleotide variation, allele frequency, and primer/probe sequences

Gene	Marker number	dbSNP ID	Chrom. pos.	Alleles	SNP location	MAF (EA)*	MAF (AA)*	Primer and probe#
CHRNA5	1	rs684513	76645455	C/G	Intron1	0.217	0.158	C_7051_10
	2	rs621849	76659916	A/G	Intron1	0.375	0.425	C_5864_10
	3	rs637137	76661031	A/T	Intron1	0.202	0.259	C_5866_10
	4	rs951266	76665596	C/T	Intron2	0.417	0.117	C_9510534_10
	5	rs17408276	76668673	C/T	Intron4	0.396	0.325	C_34624626_10
	6	rs16969968	76669980	A/G	Exon5	0.424	0.205	C_26000428_20
	7	rs615470	76673043	C/T	3′ UTR	0.325	0.342	C_18757_10
CHRNA3	8	rs578776	76675455	C/T	3′ UTR	0.242	0.342	C_721253_10
	9	rs6495307	76677376	C/T	Intron5	0.345	0.307	C_1713433_10
	10	rs1051730	76681394	C/T	Exon5	0.4	0.11	C_9510307_20
	11	rs3743078	76681814	C/G	Intron4	0.203	0.333	C_27472090_10
	12	rs1317286	76683184	A/G	Intron4	0.41	0.3	C_9510308_10
	13	rs12914385	76685778	C/T	Intron4	0.43	0.198	C_12106059_10
	14	rs2869546	76694400	C/T	Intron4	0.333	0.322	C_15836855_10
	15	rs6495308	76694711	C/T	Intron4	0.23	0.34	C_222570_10
	16	rs3743075	76696507	A/G	Exon4	0.317	0.442	C_27472089_10
	17	rs8040868	76698236	C/T	Exon2	0.457	0.364	C_261698_10
	18	rs6495309	76702300	C/T	Promoter	0.2	0.25	C_30730895_10
CHRNB4	19	rs1948	76704454	C/T	3′ UTR	0.267	0.117	C_11941837_10
	20	rs12441088	76715319	G/T	Intron1	0.25	0.3	C_30730878_10
	21	rs3813567	76721606	C/T	Promoter	N/F	N/F	C_27488001_10
	22	rs11637890	76722474	C/G	Promoter	0.347	0.067	C_31121610_10

Notes:

^*Based on the allele frequency from the NCBI SNP database

^#ID of primer/probe provided by Applied Biosystems Inc.