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. 2010 Jul 16;29(16):2788–2801. doi: 10.1038/emboj.2010.156

Figure 1.

Figure 1

Global telomeric G-overhangs undergo cell cycle-regulated dynamics regardless of telomerase expression. (A–C) HeLa cells. (D–F) IMR90 cells. (A, D) FACS analysis of DNA content in cells synchronized at the G1/S boundary and then released to S phase. Cells were collected at indicated time points for FACS analysis to determine the DNA content. A portion of IMR90 cells were permanently arrested at G0 and exited the cell cycle. (B, E) Measurement of G-overhang sizes using the overhang protection assay. The smears represent the heterogeneity of overhang sizes. ExoI digests 3′ overhangs and the ExoI plus (+) lanes show the background. The ∼54 nt band has been shown to be non-specific (Chai et al, 2005) and is not eliminated in the quantitations because its contribution to the overhang size is minimal. (C, F) Quantitation of the weighted mean lengths of G-overhangs at indicated time points during the cell cycle from (B, E). Results are representative of at least three independent experiments. Error bars represent s.d.