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. 2010 Jul 16;29(16):2788–2801. doi: 10.1038/emboj.2010.156

Figure 4.

Figure 4

The last nucleotide at C-strand is specifically defined throughout the cell cycle. (A) Scheme of STELA. (B) The specificity of the last nucleotide at C-strand determined by STELA with DNA isolated at different time points during the cell cycle from synchronized HeLa cells. The same set of DNA in Figure 1B was used. (C) Randomization of the last C in HeLa with partial depletion of Pot1. HeLa was transfected one time with Pot1 siRNA. Cells were collected 72 h after transfection for western blot and STELA. Percent of depletion is indicated below the blot.