Figure 6.

PTEN lipid phosphatase activity is required for LTD. (A–C) LTD was induced in CA1 hippocampal neurons pretreated with the PTEN inhibitor bpV(HO)pic (A) or expressing the PTEN point mutants C124S (B) or G129E (C). The results from control neurons (uninfected or untreated) were carried out in an interleaved manner with their corresponding infected or treated neurons. Amplitude of the synaptic responses is normalized to a 10 min baseline. Insets: sample traces averaged from the baseline (thin lines) or from the last 10 min of the recording (thick lines). (D) Average of AMPAR-mediated responses collected from the last 10 min of the recording and normalized to the baseline. Left columns (LTD, paired) correspond to the stimulation pathway in which postsynaptic depolarization (−40 mV) was paired to low-frequency stimulation (1 Hz). Right columns (control, unpaired) correspond to the pathway that was not stimulated during depolarization; ‘n' represents number of cells.