Table 1.
Protein quantities measured from the RCL2/CS100-fixed and frozen colonic mucosa, prostate, and breast tissues using three protein extraction protocols.
| Buffer | Colonic mucosa | Prostate | Breast | |||
|---|---|---|---|---|---|---|
| RCL2 | Frozen | RCL2 | Frozen | RCL2 | Frozen | |
| A | 1.08 ± 0.11 (43%) |
0.83 ± 0.13 (39%) |
0.80 ± 0.11 (41%) |
1.46 ± 0.02 (83%) |
1.28 ± 0.21 (56%) |
0.89 ± 0.15 (51%) |
| B | 2.36 ± 0.27 (94%) |
2.19 ± 0.38 (103%) |
1.65 ± 0.31 (84%) |
1.87 ± 0.32 (106%) |
1.86 ± 0.15 (82%) |
2.03 ± 0.24 (117%) |
| C | 2.51 ± 0.59 (100%) |
2.13 ± 0.22 (100%) |
1.95 ± 0.15 (100%) |
1.76 ± 0.17 (100%) |
2.28 ± 0.24 (100%) |
1.74 ± 0.30 (100%) |
Protein concentrations are μg/μl ± SD. Values in brackets are the percentage of protein quantity compared to buffer C, which was defined as 100%.