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. Author manuscript; available in PMC: 2011 Aug 24.
Published in final edited form as: Biochemistry. 2010 Aug 24;49(33):7202–7209. doi: 10.1021/bi100147w

Figure 1.

Figure 1

Changes in the steady-state intrinsic fluorescence intensity of M1-PYK induced by amino acids and PEP. Panels are labeled by the amino acid type (2-AB is 2-aminobutyric acid). On the left of each data panel free M1-PYK was titrated with ligand 1 (L1), where L1 was either amino acid (○) or PEP (■). On the right of each panel M1-PYK previously saturated with L1 was titrated with ligand 2 (L2). When L2 was amino acid and M1-PYK was pre-saturated with 10mM PEP, data is represented by (□). When L2 was PEP and M1-PYK was pre-saturated with amino acid, data is represented by (●); concentrations of amino acids used in these titrations were: 96 mM phenylalanine (solubility limited, is not completely saturating in the presence of PEP, see Figure 2), 25 mM 2-aminobutyric acid, 30 mM alanine, and 40 mM serine.