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. Author manuscript; available in PMC: 2010 Aug 20.
Published in final edited form as: Cell. 2010 Mar 5;140(5):652–665. doi: 10.1016/j.cell.2010.01.007

Figure 3. hnRNP E2:miR-328 Interaction Does Not Associate with the RISC Loading Complex.

Figure 3

Western blots show levels of Dicer, TRBP2, Ago2, and/or hnRNP E2 in cytoplasmic lysates (input; lanes 1 and 4), in anti-hnRNP E2 (A) and anti-Dicer (B) IPs (lane 3), in IPs with an isotype-matched IgG (lane 2), and in hnRNP E2-and Dicer-immunodepleted (ID) lysates (lane 5) of 32D-BCR/ABL-miR-328cells. (C) RIP assay for miR-328 on anti-Dicer (lane 4), anti-hnRNP E2 (lane 5), and anti-Ago2 (lane 7) IPs from 32D-BCR/ABL-miR-328 cell lysates. Note that the anti-hnRNP E2 RIP for miR-328 was performed on Dicer-immunodepleted lysates. RIP with a nonrelated IgG served as a negative control.