Dysregulated gp1 mGluR-dependent p110β expression in Fmr1 KO. A, B, DHPG treatment (15 min, 50 μm) increases p110β protein levels in WT SNS (A, normalized to control; n = 6, *p = 0.008, paired t test) and leads to a shift of p110β mRNA into puromycin-sensitive fractions (B, percentage of total mRNA in polysomal fractions; n = 6, *p = 0.028, paired t test). C, D, In Fmr1 KO SNS, DHPG treatment does not increase protein expression (C, normalized to control, n = 5, p = 0.613, paired t test) or enhance association of p110β mRNA with puromycin-sensitive polysomes (D, percentage of total mRNA in polysomal fractions, n = 3, p = 0.21, paired t test).