Figure 5.

Expression of ALDH3B1 in normal and cancerous colon. IHC was performed using antibody against human ALDH3B1 on normal human colon (A) and colonic adenocarcinoma (C,E). (A) In normal human colon, glandular epithelial cells (arrow) and cells of the connective tissue, adipose tissue, arteries, and lymphatics lack immunoreactivity. In colonic adenocarcinoma, glandular epithelial cancer cells are ALDH3B1-immunoreactive, including well-differentiated (C, arrow) and moderately differentiated (E, arrow), invasive tumors. (B,D,F) Corresponding cells from negative controls in which IHC of normal and cancerous human colon was performed using antibody against human ALDH3B1 preadsorbed with peptide show no immunoreactivity (arrows). Bar = 50 μm.