Figure 3.

Anti-pCFTR antibodies detected an ∼170-kDa protein on immunoblots of transiently transfected HEK 293 cells. Antibody pCFTR-C-1 detected a specific band between 150 and 170 kDa (asterisks). This band was not detected in lysates from vector-alone-transfected cells (mock-transfected) or when the respective preimmune serum was used (pCFTR-C-1 pre), and the band disappeared when the antibody was preincubated with the respective peptide (pCFTR-C-1 preinc.). Immunopurified antibody pCFTR-C-1/2-p detected a similar protein between 150 and 170 kDa. All immune sera were diluted 1:1000.