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. 2010 Sep;58(9):785–797. doi: 10.1369/jhc.2010.955377

Figure 4.

Figure 4

Broad tissue expression of pCFTR mRNA detected by RT-PCR. (A) Strong expression of pCFTR mRNA was detected in the upper airways (Lanes 1–7), the heart (Lane 8), the glandular part of the stomach (Lane 11), all segments of the intestine (Lanes 12–17), and the gall bladder (Lane 21). pCFTR mRNA expression was also strongly detected in the uterus, testicle, and epididymis (not shown) and weakly detected in the parotid gland (Lane 9), the esophagus (not shown), the non-glandular part of the stomach (Lane 10), the urinary bladder (Lane 20), and the pancreas (not shown). All other organs were negative, including the brain, the kidney, and all three regions of the skin. A fragment of the mRNA coding for the housekeeping gene EF-1a was RT-PCR–amplified to control for RNA integrity and efficacy of reverse transcription. The RT-PCR results shown are representative for all animals examined. Lane 22: No template control. (B) Laser capture microdissection of bronchial epithelial cells (left panel) and submucosal glands (right panel). Cells are shown before (upper panel) and after (lower panel) dissection. Bar = 50 μm. (C) pCFTR mRNA was consistently detected by RT-PCR in bronchial epithelial cells and submucosal gland cells.