Figure 6.

pCFTR is located apically to the Golgi apparatus and occasionally in the apical cell membrane. Using immunofluorescent detection on formalin-fixed, paraffin-embedded tissues, the pCFTR protein was detected in the cytosol of crypt epithelial cells, including goblet cells and non-goblet cells. In the majority of cells, the pCFTR protein (red) was detected apically to the Golgi marker 58K (green). Punctate signals were occasionally detected in or directly beneath the apical plasma membrane (arrows). Left panel, crypt epithelial cells in the colon; right panel, higher magnification of single cells; yellow dots, apical cell membranes; asterisks, locations of the nuclei. Antibody pCFTR-C-1, 1:100 dilution. Bar = 20 μm.