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Primers used for specific pCFTR amplification
| Primer sequences | Position in pCFTR sequence (GenBank accession number NM_001104950) | Amplicon length (bp) |
|---|---|---|
| 5′-CCTGGGAAGCTTGATTTTG-3′ (upstream) | nt 4000–4018 | 450 |
| 5′-CTAAAGTCTTGTTTCTTGC-3′ (downstream) | nt 4431–4449 | |
| 5′-AACCTGAACAAGTTTGATGAAG-3′ (upstream) | nt 559–580 | 128 |
| 5′-AAGGCAAGTCCACAGAAGGC-3′ (downstream) | nt 667–686 |
pCFTR, porcine cystic fibrosis transmembrane conductance regulator.
