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. 2010 May 12;83(3):464–472. doi: 10.1095/biolreprod.109.083238

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© 2010 by the Society for the Study of Reproduction, Inc.

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FIG. 4. — Localization at acrosomal proteins (zonadhesin, proacrosin, SPAM1/PH-20, CST8 [CRES], and ZP3R/sp56) in spermatozoa. Using Cstf2t^+/+ (A) and Cstf2t^−/− (B) spermatogenic cells, the first column of the panels in A and B shows sperm cells from the cauda in phase contrast. The same sperm cells were labeled with an antibody to the protein described in the left margin (middle column), and the last column of images shows the same previous spermatozoa but labeled that time with PNA lectin. Note that each spermatozoon was positive for the PNA lectin and for the five sperm antibodies, with the exception of the negative control (GST or CREM, irrelevant antibody to sperm cells). Original magnification ×96.