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. Author manuscript; available in PMC: 2011 Aug 6.
Published in final edited form as: J Proteome Res. 2010 Aug 6;9(8):4215–4227. doi: 10.1021/pr1005197

Figure 1. LC-MRM Screening to Select Peptides for Assay Development to Monitor Wnt/β-catenin Signaling Components and Downstream Protein Expression.

Figure 1

A GeneGO pathway map is shown to illustrate the interactions between the selected proteins. The results of LC-MRM screens used to select peptide candidates for quantitative assay development are shown for four selected proteins, β-catenin, c-Src, c-Myc, and the catalytic subunit of PP2A. Each extracted ion chromatogram plot shows peptide signal over time. Using these data, one peptide standard is developed for quantification, but all peptides detected in these screens are monitored in biological experiments. The Roman numerals link to the peptide sequences listed in Table 1.