Table 2. Limits of Detection and Quantification for Selected Peptides from β-Catenin, c-Src, c-Myc and PP2A in SW480 Colon Cancer Cells.
Peptides in bold font indicate the best sequences for monitoring the corresponding proteins based on the signal intensity and consistent data acquired from the triplicate LC-MRM experiments.
| Protein | Peptide | Limit of Detection (Cells) | Limit of Quantification (Cells) |
|---|---|---|---|
| β-catenin | AIPELTK (II) | 100 (S/N 411 ± 147) | 1000 |
| LLNDEDQVVVNK (III) | 500 (S/N 61 ± 11) | 1000 | |
| SGGIPALVK (IV) | 500 (S/N 128 ± 30) | 1000 | |
| LHYGLPVVVK (VI) | 5000 (S/N 136 ± 30) | 5000 | |
| LLWTTSR (VII) | 100 (S/N 33 ± 8) | 100 | |
| LVQLLVR (VIII) | 500 (S/N 72 ± 17) | 1000 | |
| EGLLAIFK (IX) | 100 (S/N 136 ± 19) | 1000 | |
| NEGVATYAAAVLFR (X) | 1000 (S/N 332 ± 95) | 1000 | |
| c-Src | EVLDQVER (I) | 5000 (S/N 58 ± 5) | 5000 |
| GPSAAFAPAAAEPK (II) | 10000 (S/N 36 ± 11) | 10000 | |
| TETDLSFK (III) | 5000 (S/N 107 ± 19) | 5000 | |
| LLLNAENPR (IV) | 5000 (S/N 20 ± 2) | 5000 | |
| LFGGFNSSDTVTSPQR (V) | 10000 (S/N 35 ± 6) | 10000 | |
| WTAPEAALYGR (VI) | 5000 (S/N 100 ± 24) | 5000 | |
| GSLLDFLK (VII) | 5000 (S/N 506 ± 75) | 5000 | |
| c-Myc | DQIPELENNEK (I) | 1000 (S/N 27 ± 7) | 5000 |
| SSDTEENVK (II) | 5000 (S/N 43 ± 17) | 5000 | |
| PP2A catalytic subunit | GGWGISPR (II) | 500 (S/N 32 ± 10) | 500 |
| YSFLQFDPAPR (III) | 100 (S/N 41 ± 1) | 100 |