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. 2010 Aug 11;7:66. doi: 10.1186/1742-4690-7-66

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Copyright ©2010 Raghavendra et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Immunoblotting for host proteins that are specifically associated with HIV-1 PIC. The proteins bound to the streptavidin magnetic beads after integration assay were probed with specific antibodies. SupT1-H9/HTLVIIIB represents HIV-1 infected cell samples, and SupT1-H9 represents non-infected control samples. The host proteins are indicated by accession names on the left. The 'NONO' is Non-POU domain-containing octamer-binding protein, 'NP1L1' is Nucleosome assembly protein 1-like 1 protein and 'CALR' is Calreticulin for which 8, 5 and 5 peptides were identified by MS analysis respectively. Beta-actin found in both samples is also shown.