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. Author manuscript; available in PMC: 2010 Aug 20.
Published in final edited form as: Brain Cell Biol. 2008 Oct 22;36(1-4):87–100. doi: 10.1007/s11068-008-9030-y

Figure 5. Recycling and resting vesicle pools interchange across boutons.

Figure 5

After assessing the size of the total vesicle pool with NH4Cl and estimating its recycling pool with a train of stimulation (a), all vesicles in a single bouton where bleached (red arrow in right panel pictures, red trace in left plots). (b-d) The same protocol applied 5 (b), 30 (c), and 160 (d) minutes after bleaching indicates that non-bleached vesicles reached the originally bleached bouton and distribute in both recycling and resting pools. (e) After 3 hours, non-bleached vesicles account for 91% of originally bleached ones (NH4Cl, compare with a); of these, 51% are capable of recycling (stimuli in Baf). The response to the same series of trials is also shown for a neighboring, non-bleached, bouton (a-e, white arrow in right panel pictures, black traces in left plots). Scale bar 5μm.