Table 1. Application of nutrient withdrawal test to LC88 cells growing on 4FTrp, 5FTrp or 6FTrp.

	Growth on 4FTrp	Growth on 5FTrp	Growth on 6FTrp
Class I: 80%–100% growth rate	A,T,C,G,U, Ala, Arg, Asn, Asp, Cys, Gly, Gln, Glu, His, Ile, Lys, Pro, Ser, Val, p-aminobenzoic acid, biotin, folic acid, niacinamide, pantothenate, pyridoxal, ribitol, riboflavin, thiamine.	T,C,G,U, Ala, Arg, Asn, Cys, Gly, Gln, Glu, His, Ile, Lys, Pro, Ser, Thr,Val, p-aminobenzoic acid, biotin, folic acid, niacinamide, pantothenate, pyridoxal, ribitol, riboflavin, thiamine	T,C,G,U, Ala, Arg, Asn, Asp, Cys, Gly, Gln, Ile, Lys, Pro, Ser, Val, p-aminobenzoic acid, biotin, folic acid, niacinamide, pantothenate, pyridoxal, ribitol, riboflavin, thiamine
Class II: 40%–79% growth rate	Thr	A, Asp	A, His
Class III: 10%–39% growth rate	Leu	Leu	Leu, Thr
Class IV: 0%–9% growth rate	Met	Met	Glu, Met

The various nutrients withdrawn one at a time from medium G are shown in columns 2–4, where the growth effect due to each withdrawal is grouped into Classes I-IV depending on the growth rate observed under the withdrawal as a percentage of the control growth rate in complete medium G.