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. 2010 Aug 20;5(8):e12325. doi: 10.1371/journal.pone.0012325

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Kemper et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Prominin-1 protein compared to the prominin-1 mRNA sequence. Indicated is where the spliced regions of the mRNA are localized on the protein. None of the N-linked glycosylation sites are localized in any the spliced exons. B. Immunoblot for prominin-1 on lysates of several mouse organs and murine CRC cell lines. As loading control, actin was used. C. Lysates of several mouse organs were deglycosylated with PNGaseF and compared to untreated controls.