Skip to main content
. Author manuscript; available in PMC: 2011 Aug 25.
Published in final edited form as: J Neuroimmunol. 2010 Jun 3;225(1-2):82–90. doi: 10.1016/j.jneuroim.2010.04.019

FIGURE 5.

FIGURE 5

Digital images obtained from a Z-stack originated with a Zeiss LSM 510 confocal microscope at 630 times magnification in layer 5 of the cerebral cortex. Consecutive images (A, C, E, G) at 1μm step between images revealing CCL27 immunostaining (red) in combination with Hoechst nuclear staining (blue). Corresponding same consecutive images are shown in B, D, F, H, revealing CCL27 immunostaining (red) and the nuclear neuronal marker NeuN (green). The arrows indicate the same position between stacks of selected neurons showing perinuclear CCL27 immunoreactivity. The image show that almost all the CCL27 signal is co-localized with neurons with the exception of one cell indicated with an arrowhead. Scale bar in G and H = 10 μm applied to all panels.