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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: J Parasitol. 2010 Aug;96(4):815–819. doi: 10.1645/GE-2406.1

FIGURE 2.

FIGURE 2

(A) Slot-blot assay using 2 μg of total RNA (top panel). mRNA depletion is observed in μ1, μ2, and rdrp induced trophozoites (I) by testing the 3′ endogenous sequences. gdh antisense probes are shown as controls. cwp2 mRNA is slightly reduced in induced encysting cells. The amount of total RNA for each sample is depicted by staining with methylene blue (bottom panel). (B) Immunoblot assays using anti-HA mAb (SIGMA), 2F5 mAb (Rivero et al., unpublished), and anti-GFP mAb (Santa Cruz Biotechnology, Santa Cruz, CA) to detect μ1-HA, μ2, and eGFP, respectively; show a substantial lower expression of these proteins in induced trophozoites compared with non-induced cells. Uniform BiP, tubulin, and CWP2 protein expression are shown using 9C9, anti-αTubulin (SIGMA), and 7D2 mAbs, respectively.